NeOnc Technologies Holdings, Inc.

NEO100-01 GBM TRIAL

Background Glioblastoma (GBM, WHO grade IV glioma) is the most common primary malignant brain tumor among adults. Regardless of the treatment regimen, many patients relapse and are faced with limited treatment options. The aggressive infiltration of GBM throughout the brain typically limits the efficacy of repeat surgical resection, and tumor cells frequently acquire resistance to […]

Background

Glioblastoma (GBM, WHO grade IV glioma) is the most common primary malignant brain tumor among adults.

Regardless of the treatment regimen, many patients relapse and are faced with limited treatment options. The aggressive infiltration of GBM throughout the brain typically limits the efficacy of repeat surgical resection, and tumor cells frequently acquire resistance to further cytotoxic therapy. Therefore, recurrent GBM does not respond well to repeat surgery, re-irradiation, and additional rounds of chemotherapy. At the same time, these interventions may moderately increase overall survival, but the prognosis for these patients remains exceptionally poor.

Given the persistent medical need for improved treatments, NeOnc began investigating a type of intervention, intranasal delivery of perillyl alcohol (POH, NEO100), for patients with recurrent GBM. Extensive preclinical studies provided strong evidence of POH’s natural anticancer potential. Though the exact mechanism of POH’s anticancer effect is unclear, it most likely results from the pleiotropic effects including cell cycle arrest, endoplasmic reticulum stress, and induction of apoptosis.

Protocol

In the two-year study conducted at the Cleveland Clinic at the University of Washington/Seattle, the University of Wisconsin, and the University of Southern California study, 12 patients were enrolled and divided into four cohorts. Candidates for the clinical study needed to meet the following criteria:

  • Were 18 years of age or older.
  • Had a confirmed progression or recurrent grade IV Glioma.
  • Had been on a stable dose of steroids for at least five days.
  • Had failed previous radiation and Temozolomide treatment.
  • Had an ECOG1 performance status of 0-2, or;
  • Had Karnofsky’s 2 performance status ≥60.
  • Had an expected survival rate of at least 3 months.
  • Showed a baseline MRI with gadolinium within two weeks of trial.
  • Had seizures which were being controlled on a stable dose of anti-epileptics for two weeks prior to
    enrollment.

The cohorts in the study were divided into four groups.  Each group was given a specific dosage of NEO100 administered by intranasal delivery using a nebulizer and nasal mask for 28 days. Dosages were 96 mg/dose (384 mg/day) for Group 1, 144 mg/dose (576 mg/day) for Group 2, 192 mg/dose (768 mg/day) for Group 3, and 288 mg/dose (1152 mg/day) for Group 4.

Patients stayed in the trial for as long as there was no disease progression.

The patients underwent gadolinium-enhanced brain MRI as part of standard care. Baseline tumor measurement was performed within two weeks of registration and assessed by RANO criteria (Response Assessment in Neuro-Oncology). MRIs were repeated after every 28-day cycle (i.e., cycles 2, 4, and 6) and whenever disease progression was suspected based on clinical symptoms. Tumor response was assessed using the MacDonald and the RANO response criteria for high-grade gliomas, which considers radiologic imaging, neurological status, and steroid dosing.

Safety was evaluated throughout the trial by the incidence of adverse events (AE), physical examination findings, vital signs, and clinical laboratory test results. AE were graded for severity using NCI Common Terminology Criteria for Adverse Events v.4.0.

Results

Phase 1 of the study showed that Intranasal NEO100 was well tolerated at all dose levels, and no severe adverse events were reported.

  • 30% of patients had no disease progression after six months
  • There was a 100% survival rate after 12 mo. with patients who received a minimum of 4 cycles
  • In one patient, there was complete remission of the disease at the two-year mark
  • Another patient who continued to receive NEO100 after the conclusion of the trial was still alive at the three-year mark
  • The overall survival rate and survival time of patients who received NEO100 was significantly higher vs.
    similar GBM studies using traditional
    chemotherapeutic drugs and delivery methods.
  • All subjects well tolerated the therapy.
  • Only minor side effects were noted, such as fatigue, headaches, and runny nose.

As a result, NEO100 demonstrated an ability to provide improved survivability when compared to historical controls. It points to the potential that this novel intranasal approach could be helpful in treating recurrent GBM.

Publishing

This part of the NEO100GBM Study concluded in 2020 and the results were published in 2021 in the Neuro-Oncology Advances Journal (NOAJ). The NOAJ is a quarterly journal under the auspices of the Oxford University Press, the largest university press in the world, and its printing history dates to the 1480s.

The study was authored by Axel Schonthal PhD3, David M Peereboom4, Naveed Wagle5, Rose Lai6, Anna J Mathew7, Kyle M Hurth8, Vincent F Simmon9, Steven P Howard10, Lynne P Taylor11, Frances Chow12, Clovis Orlando da FonsecaNeOnc Technologies, Inc., Los Angeles, California and Department of General and Specialized Surgery, Antonio Pedro University Hospital, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil[/efn_note], and Dr. Thomas C Chen13